Effect of cocaine-amphetanmine-tegulated transcript peptide on the content of 4-hydroxy-2-nschenfia/reperfusion in ndce

Objective To investigate the effect of cocaine-amphetamine-regulated transcript peptide （CART） on the content of4-hydroxy-2-noneral （HNE） and infarct volume after cerebral ischemiaJreperfusion in mice. Methods A total of 96 healthy male mice were randomly divided into four groups： ischemla/reperfusion （n =27）, CART （n =27）, normal saline control （n =27） and sham operation （n = 15） groups. A middle cerebral artery occlusion （MCAO） model was induced. Two hours after MCAO, CART 55-102 and equivalent normal saline were injected respectively via the tail veins of mice in the CART group and the normal saline control group, and then they were injected every other 24 hour. The neurological scores, infarct volume and the HNE content of lipid metabolism of oxidative stress were performed and detected respectively at 12, 24, 48 and 72 hours after reperfusion. Results CART could significantly improve the neurological deficit scores （all P 〈0. 05） and reduce infarct volume （all P 〈0. 05） at different time points after ischemia/reperfusion. The content of liNE was upregulated （all P 〈0. 05） at different points after referfusion. CART could significantly down-regulate the increased HNE level in brain after ischemia （all P 〈 0. 05）. Conclusions CART may protect ischemic brain injury in mice by inhibiting lipid peroxidation. Key words: Cocaine- And Amphetamine-Regulated Transcript Protein;  Brain Isehemia;  Stroke;  4-Hydroxy-2-Nonenal;  Neuroprotective Agents;  Miceoneral and infarct volume after cerebral i