Determination Of Sorafenib In Spiked HumanUrine By Differential Pulse PolarographyAt Dropping Mercury Electrode

The electrochemical reduction behavior and determination of sorafenib was studied by differential pulse polarography at dropping mercury electrode. A linear response was obtained over the concentration range 5.0x10-8 to 1.0x10-5M with lower detection limits 4.2x10-8M for sorafenib.Sorafenib exbits well defined cathodic waves in universal buffers over the pH range 2.0 to 6.0. The carbonyl group getting reduced to the saturated compound in a four electron processes and reduction mechanism has been proposed. The kinetic parameters such as diffusion coefficients (D), transfer coefficients (αna) and heterogeneous forward rate constants (K0fh) are evaluated and reported. The relative standard deviation and correlation coefficient value was found to be 0.326% and 0.65 respectively. Differential pulse polarography was employed for determination of the sorafenib in trace levels using both standard addition and calibration methods.