Diversity of Endophytic Fungi Isolated from Gentiana rhodantha and Screening for Biocontrol Stains

In order to clarify the composition of endophytic fungi in Gentiana rhodantha  in colonization tissues with four Liupanshui sampling sites (Fenghuang Zhongshan, Nankai Shuicheng, Yangmei Shuicheng and Xinchang Liuzhi) in Liupanshui, strains were isolated with health leaf, stem and root and identification based on morphological characteristics and ITS sequence analysis. As well as screening for biocontrol stains by the dural culture method on PDA plates with Sclerotinia sclerotionrum . The results showed that  G. rhodantha  concealed rich fungal diversity, the colonization rate and isolation rate of endophytic fungi reached 49.38% and 61.25% respectively. 294 strains of endophytic fungi were obtained from 480 explant tissue segments of G. rhodantha  and belonging to 2 phylum, 5 classes, 14 orders and 32 genera; of which Fusarium  and Colletotrichum  were comparatively advantageous taxa in endophytic fungi of G. rhodantha , and the species contained occupied 9.86% and 8.84% of the total endophytes respectively．According to the calculation results, Xinchang LZ in four sites and stem in three tissues showed optimum degrees of diversity, evenness and dominance respectively. 23 strains of endophytic fungi with inhibitory activities against S. sclerotionrum  by the dural culture method; Amomg them, the inhibition rate of Botryosphaeria  sp. R022, R021, Phomopsis sp. S002, Colletotrichum sp. S028 and Fusarium sp. L031 were higher than 70% and showed the best inhibitory potential.