Ellagitannin and flavonoid constituents from Agrimonia pilosa Ledeb. with their protein tyrosine phosphatase and acetylcholinesterase inhibitory activities

Abstract A new ellagitannin, agritannin ( 1 ), a new flavone glycoside, agriflavone ( 2 ), and another flavone glycoside with spectroscopic data reported for the first time, kaempferol-3- O -[( S )-3-hydroxy-3-methylglutaryl (1→6)]- β - d -glucoside ( 3 ), along with 16 known compounds were isolated from the aerial parts of Agrimonia pilosa Ledeb. These compounds were evaluated for PTP1B inhibitory activity. Among them, compounds 9 and 18 displayed potential inhibitory activity against PTP1B with IC 50 values of 7.14 ± 1.75 and 7.73 ± 0.24 μM, respectively. In addition, compound 1 showed significant inhibitory effect with an IC 50 value of 17.03 ± 0.09 μM. Furthermore, these compounds were tested in AChE inhibitory assays. Most of them were found to have moderate inhibitory effects, with IC 50 values ranging from 60.20 ± 1.09 to 92.85 ± 1.12 μM. Except compounds 3 , 8 , and 18 were inactive.