Three-Domain Antibodies against the Tumor Necrosis Factor: Investigation of Their Biological Activity In Vitro

The aim of this work was to evaluate novel three-domain antibodies consisting of two domains specific for human tumor necrosis factor (hTNF), and of the third domain responsible for binding to myeloid cells. The additional hTNF-binding domain should serve to increase the biological activity of new antibodies. Capacity of these proteins to bind hTNF on the macrophage surface and to neutralize its biological activity in vitro was assessed.