Induction of Microspores-derived Embryos and Calli from Anther Culture in Carrot

The influence of genotype,hormone,AgNO3 and low temperature (4 ℃) on anther culture of carrot (Daucus carota L.) was investigated with 7 open pollinated cultivars,10 inbred lines,16 F1 and 6 F2.Microspore embryogenesis occurred on six accessions and the highest frequency was 50071 with 3.89%.Thirty accessions formed the calli derived from microspores and 600Q6 had the highest frequency with 36.70%.On the B5 media with 2,4-D and NAA,all of four tested accessions formed the calli,and both of 50071 and 70Q67 formed the microspores-derived embryos,but none on the media with 6-BA and NAA.50071 and 70Q67 were induced about 10.00% and 2.00% of embryos on the media with 8 mg · L-1 AgNO3,respectively.Low temperature was effective on some accessions to form embryos and calli.50069 had the embryo ratio of 1% under the treatment of 1 d,and the callus ratio of 11.25% under the treatment of 2 d and 3 d,respectively.70Q67 had the embryo ratio of 4.00% with 3 d treatment.About 93.39% plants were diploid among 121 plantlets regenerated from embryos with cytogenetic analysis,which was regarded as the result of spontaneous diploidization during anther culture.