[Determination of vetatramine in Veratrum nigrum by HPLC-ELSD].

Objective:To develop an HPLC-ELSD method for determination of vetatramine.in Veratrum nigrum.Method:The analy fical column was Shim-pack ODS-C18(4.6 mm×250 mm,4 μm)column,the mobile phase was acetonitrile-water(containing 0.1% triethylamine)(50∶50),at a flow rate of 0.8 mL·min-1.The temperature of drift tube was 90 ℃ and the gas flow was at the rate of 2.5 L·min-1.Result:The calibration curve was linear in the range of 0.36-3.6 μg(r=0.999 8).The average recovery was 100.9%(RSD 2.3%,n=6).The contents of veratramine in Veratrum nigrum.from the ten different sources were determined.Conclusion:The method may be used as a accurate and reproducible way to determine the content of veratramine in V.nigrum.