Chemical Complementation Assays

Originally published in: Enzyme Assays. Edited by Jean-Louis Reymond. Copyright © 2006 Wiley-VCH Verlag GmbH & Co. KGaA Weinheim. Print ISBN: 3-527-31095-1 The sections in this article are Introduction Complementation Assays Introduction Early Complementation Assays Enzymology by Complementation Directed Evolution by Complementation Development of Chemical Complementation Introduction Three-hybrid Assay Original Yeast Three-hybrid System Dexamethasone–Methotrexate Yeast Three-hybrid System Technical Considerations Other Three-hybrid Systems Chemical Complementation Selection Scheme and Model Reaction Results General Considerations Related Methods Applications of Chemical Complementation Introduction Enzyme-Inhibitor Interactions Rationale Screen Strategy Enzyme Library Screen General Considerations Glycosynthase Evolution Rationale Selection Scheme Glycosynthase Assay Directed Evolution General Considerations Conclusion Keywords: enzyme assays; chemical complementation; complementation assays; development of chemical complementation; application; three-hybrid assay; enzyme-inhibitor interactions