Quantitative Determination of Ferulic Acid Content in Chrysanthemum Morifolium cv. (Chuju) Continuous Cropping Soil Using Near Infrared Spectroscopy

Abstract A near infrared spectroscopy (NIRS) method was used for rapid quality evaluation of ferulic acid content in chrysanthemum morifolium cv . (Chuju) continuous cropping soil. Standard leverage, studentized residual and Mahalanobis distance were calculated to eliminate abnormal samples. After the initial near infrared spectrum was treated by two second derivative and Norris smoothing filter noise, 6000–4000 cm −1 wave number range and 7 factors were chosen for partial least squares (PLS) calibration model. The results showed that good correlations were presented between the calibration set/validation set and the HPLC values, and the calibration correlation coefficient ( R c ) and validation correlation coefficient ( R cv ) were 0.9914 and 0.9935. Root mean square error of calibration (RMSEC), root mean square error of prediction (RMSEP) and root mean square error of cross prediction (RMSECV) were 0.484, 0.539 and 0.615, respectively. This method was accurate, simple, rapid, nondestructive, and suitable for analysis of ferulic acid in continuous cropping soil.