Immunoprecipitation experiments with commercial antibodies

Copyright information: Taken from "A simplified immunoprecipitation method for quantitatively measuring antibody responses in clinical sera samples by using mammalian-produced luciferase-antigen fusion proteins"BMC Biotechnology 2005;5():22-22.Published online 18 Aug 2005PMCID:PMC1208859.Copyright © 2005 Burbelo et al; licensee BioMed Central Ltd. Various amounts of anti-FLAG monoclonal, anti-p53 polyclonal or control (anti-WASP) polyclonal antibodies were mixed with 5 μl of a Cos1 extract containing Ruc-p53 for 1 h in the presence of protein A/G beads, processed and light units measured. The data shown is from one of three independent experiments giving similar results.