Death in the clouds: ranavirus associated mortality in assemblage of cloud forest amphibians in Nicaragua

Amphibian diseases are acknowledged as significant contributors to the decline and extinction of amphib- ian species. The main culprits currently considered are chytridiomycosis and Ranavirus. In Central America, highly endemic and geographical restricted terrestrial species may be at risk from these diseases. We collected 49 Agalychnis callidryas larvae, one Lithobates forrei and five unidentified larvae on the Nicaraguan Island Ometepe, all deceased, and skin samples were taken. The presence of Ranavirus was determined using PCR. Ranavirus was found involved in 41 of 55 tadpoles. Forty-one Agalychnis callidryas , one Lithobates forrei and another five unidentified anuran tadpoles.