DNA microarray analysis of expression of growth phase-responsive genes in antibiotic-producing Streptomyces sp. KH29

Screening of new compounds and elucidation of biosynthetic pathways for secondary metabolites are very important fields of biology; however, most of the currently identified compounds have already been well-researched. Here, we set out to identify genes related to secondary metabolite production, specifically antibiotic production, by Streptomyces sp. KH29, using known DNA chip data for S. coelicolor. Our results indicate that the relationship between the growth-phase–dependent production of antibiotic against multi-drug resistant A. baumannii and the expression profiles of antibiotic biosynthetic gene expression profiles could lead to a rational strategy for improvement of actinomycetes genetic strains. Furthermore, genome-wide screening using cDNA microarrays containing sequences from the S. coelicolor genome, together with industrial strains that over-produce antibiotics and are related to the Streptomycetes, and may be an efficient approach for the discovery of regulatory genes affected by unidentified mutations in the industrial strains.   Key words: Streptomyces sp. KH29, biosynthetic genes, DNA microarray