Effect of carpesium abrotanoides petroleum ether fraction on phagocytic function of macrophages and antibody forming cell in mice

Objective To study the effects of carpesium abrotanoides petroleum ether fraction on phagocytic function of macrophages and antibody forming cell in mice. Methods The extract carpesium abrotanoides petroleum ether fraction, forty five mice were divided into the control group and low and high dose groups of carpesium abrotanoides petroleum ether fraction by random number table method, 15 mices in each group. The low-dose and high-dose groups of carpesium abrotanoides petroleum ether fraction were given 50 mg/kg and 100 mg/kg respectively by gavage for 10 days. The spleen weight, growth index, the phagocytosis of macrophages and the number of antibody-forming cells in peritoneal macrophages of mice were detected by chicken erythrocyte phagocytosis test and hemolytic plaque test. Results Compared with the control group, the spleen weight (192.4 ± 11.49 mg, 204.6 ± 10.59 mg vs. 117.6 ± 10.89 mg), the growth index (6.04 ± 0.54, 6.06 ± 0.40 vs. 3.89 ± 0.14), antibody forming (1 216.4 ± 94.1, 1 548.8 ± 86.4 vs.361.0 ± 11.7), phagocytosis percentage of macrophages (58.60% ± 2.60%, 72.0% ± 3.08% vs. 35.49% ± 1.64%), and Phagocytosis index (2.01 ± 0.10, 2.69 ± 0.15 vs. 0.37 ± 0.06) of the groups of low doses and high doses of Carpesium abrotanoides Petroleum ether fraction significantly increased (P<0.05). Conclusions The low doses and high doses of Carpesium abrotanoides Petroleum ether fraction can enhance the mice's specific and nonspecific immune function,and protect the immune system of mice. Key words: Carpesium abrotanoides; Macrophages; Immune function; Mice