Association of -160A/C single nucleotide polymorphism in E-cadherin gene promoter region with colorectal cancer in China.

Objective:To investigate the association of-160A/C polymorphism located in the CDH1 gene promoter region with risk of developing colorectal cancer(CRC) in China.Methods: The case-control study was designed,based on the population of colorectal cancer in China and healthy normal controls who have no tumor or inherited disease history.Polymerase chain reaction-restriction frag-ment length polymorphism(PCR-RFLP)was used to genotype the variant,and immunohistochemical staining was performed to measure the expression of E-cadherin in different allele cases among the CRC patients and normal controls.Results: There was no significant difference in the genotype frequencies of the polymorphism between normal controls and CRC patients(x2test,P=0.602),there was no significant difference in E-cadherin expression for the A-allele and C-allele in normal controls and CRC(t-test,P 0.05).Conclusion: The-160A/C promoter polymorphism of CDH1 gene may play no role in the etiology of CRC independently.