Effect of Different Explants Treatments on in vitro Plant Regeneration of Pepper(Capsicum annuum L.)

The hypocotyl segments of pepper seedlings,seedlings without capitals,seedlings decapitated after wounded by puncturing the hypocotyls,the half seeds consisting of the proximal part of the hypocotyls and the radicles,and the half seeds consisting of the cotyledons,the apical part of the hypocotyls and the embryos of 5 inbred lines(0510,0516 and 7013 were sweet pepper,0818 and 0819 were hot pepper)of pepper(Capsicum annuum L.)were used as explants for regeneration.MS medium with or without PGSs(plant growth substances)was used for culturing.There was not any regenerant obtained from explants cultured in the medium with PGSs,while some regenerate shoots emerged from explants cultured on the PGSs-free medium,of which the average regenerate rate of seedlings decapitated when the cotyledons did not exposure yet(about 7 days after sowing)and seedlings decapitated at the