Comparative proteomics study of dl-3-n-butylphthalide protecting PC12 cells from hypoxia-induced damage

Objective To explore the protective effect and molecular mechanism of dl-3-n-butylphthalide (NBP) against oxygen-glucose deprivation induced by sodium hydrosulfite in PC12 cells at proteomic level.Methods PC12 cells impaired by serum-free culture media with sodium hydrosulfite were used as the cell models ofhypoxia,and the protective effects of NBP on hypoxic cells were observed.Methyl thiazolyl tetrazolium (MTT) assay was used to measure the viabilities of PC12 cells.Proteomic technique was employed to identify the differential expression proteins.Results Following the increment of NBP concentrations,the cell absorbance (A) value increased gradually (the PC12 cell apoptosis reduced gradually); when the NBP concentration reached 5 mol/L,their cell A value was significantly different as compared with that of cells without adding NBP (P＜0.05).By using proteomics methods,17 differentially-expressed protein spots in the cells without adding NBP and cells with NBP were identified; most of proteins were cytoskeleton proteins,apoptosis related proteins and oxidative stress related proteins.Conclusion NBP can reduce neuronal apoptosis induced by hypoxia,and cytoskeletal proteins,apoptosis-related proteins and oxidative stress related proteins may role in protection of NBP on ischemic neurons. Key words: D1-3-n-butylphthalide; PC12 cell; Hypoxia; Sodium hydrosulfite; Proteomics