Optimization of Cultivation Conditions for Production of Fructosyltransferase by

The investigation of optimal conditions for the fructosyltransferase (FTase) production by Aureobasidium pullulans CCY 27-1-94 was carried out in shaken flasks. The eect of four medium components was investigated by varying their content compared to the reference medium composition. It was found that none of the investigated components had a very strong eect either on the cell growth or the FTase production. It was, however, disadvantageous to decrease the content of the components below the values in the reference medium. The influence of initial sucrose concentration and cultivation time was investigated in the second stage using a rotatable central composite design where the sucrose concentration was 208|491 g dm 3 and the cultivation time was from 26 to 94 h. The sucrose content did not influence the total production of FTase but high sucrose concentrations resulted in high specic activities of the cells. The cultivation time had a positive eect on the fructosyltransferase production up to 40|60 h but it decreased the specic enzyme activity. The eect of the composition of the cultivation medium on the total costs of fructooligosaccharide production was also evaluated.