Comparison of fluorescent PCR method and bacterium culture method in Salmonella typhimurium food poisoning detection

Objective:To clear whether fluorescent PCR method could improve the detection rate of Salmonella Typhimurium by comparison with traditional bacterium culture method.Methods: Test 11 samples by using traditional bacterium culture method and fluorescent PCR method respectively.Results: 6 Salmonella Typhimurium(not typed) are detected by fluorescent PCR method among 11 samples with a positive rate of 54%,while 4 Salmonella Typhimurium are detected by bacterium culture method with a positive rate of 36%.Conclusion: Compared to the conventional bacterium culture method,fluorescence PCR method could increase the detection rate of Salmonella Typhimurium,shorten the detection time,and it is suitable for rapid diagnosis of food poisoning.