Two New N-(O-Carbamoylglucopyranosyl)-N-dimethylansamitocins from Actinosynnema pretiosum

Two new and a known N-(O-carbamoylglucopyranosyl)ansamitocins were isolated from Actinosynnema pretiosum ssp. auranticum ATCC 31565. The known N-(4-O-carbamoyl-beta-D-glucopyranosyl)-N-demethylansamitocin P 2 (=ACGP-2; 1) was assigned according to 1D- and 2D-NMR data, and the two new compounds were identified as N-(6-O-carbamoyl-beta-D-glucopyranosyl)-N-demethylansamitocin P 2 (=ACGP-2'; 2) and N-(4-O-carbamoyl-beta-D-glucopyranosyl)-N-demethylansamitocin P 1 (=ACGP-1; 3) on the basis of spectroscopic data interpretation including 2D-NMR and tandem MS analysis.