Rumen fluid transplantation affects growth performance of weaned lambs by altering gastrointestinal microbiota, immune function and feed digestibility

Although rumen fluid transplantation (RT) has been developed to confer benefits for adult ruminants by altering gastrointestinal tract microbiota, the question remains whether RT can also benefit weaned lambs. Hence, in this study, thirty-eight pre-weaning lambs were randomly assigned to one of three treatment groups: control lambs (CON) received 25 ml of normal saline solution, and lambs in two RT groups received 25 ml of rumen fluid either from 3-month-old lambs (LT) or from one-year-old adult ewes (AT). The effects on their growth performance, nutrient digestibility, some blood parameters and gastrointestinal tract microbiota were monitored. There were differences (P 0.05), but a bacterial community change was observed after RT, and the abundance of some dominant bacteria in both rumen and rectum changed after RT (P < 0.05). Analysis of correlations between the parameters indicated that the altered gastrointestinal microbiota and accelerated maturity of rumen microorganisms induced by RT caused some impairment of gastrointestinal integrity and immunity, which led to decreased feed intake, reduced feed digestibility and lower growth performance of the weaned lambs. In conclusion, rumen fluid transplantation altered the gastrointestinal microbiota causing adverse effects on feed intake, feed digestibility and growth performance of the weaned lambs.