Recombinant pichia pastoris for preparing hyaluronic acid and construction method of recombinant pichia pastoris

The invention discloses recombinant pichia pastoris for preparing hyaluronic acid and a construction method of the recombinant pichia pastoris, belonging to the technical field of bioengineering. According to the invention, hyaluronic synthase hasA derived from streptococcus zooepidemicus and UDP-glucose dehydrogenase tuaD derived from bacillus subtilis are adopted and are respectively expressed under constitutive promoters GAP and TEF1, and hyaluronic acid is prepared in pichia pastoris GS115. The recombinant pichia pastoris lays a certain foundation for efficiently preparing hyaluronic acid and is suitable for industrial production application.