Gene Expression Profiling Related to the Enhanced Erythropoiesis in Mouse Bone Marrow Cells

Peroxiredoxin II knockout (Prdx II−/−) mice had a spontaneous phenotype of hemolytic anemia. In this study, we found that Ter-119+CD71+ cells increased in Prdx II−/− mice bone marrow (BM) at 8 weeks of age. We examined the differential expression profiles to bone marrow cells (BMCs) between Prdx II+/+ and Prdx II−/− mice using a cDNA microarray. We identified the 136 candidates were differentially expressed a greater twofold increase or decrease than EPO receptor. In this study, we focused on the up-regulated NBPs during erythropoietic differentiation. According to cDNA microarray results, six NBPs except zfp-127 were up-regulated during erythropoiesis in Prdx II−/− mice. Among the six candidates, eIF3-p44, hnRNPH1, G3bp, and Zfpm-1 were dramatically increased at day 7 of the in vitro erythropoietic differentiation of human CD34+ cells. However, DJ-1 and Rbm3 were slightly increased only at day 12. Our results suggest that up-regulated NBPs might be involved during erythropoietic differentiation. J. Cell. Biochem. 104: 295–303, 2008. © 2007 Wiley-Liss, Inc.