beta-Hydroxybutyrate dehydrogenase decorated MXene nanosheets for the amperometric determination of beta-hydroxybutyrate.

MXene nanosheets of type Ti3C2Tx were modified with beta-hydroxybutyrate dehydrogenase and then used as a biosensor for amperometric sensing of beta-hydroxybutyrate. The MXene and the nanocomposite were characterized by X-ray photoelectron spectroscopy, field-emission scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. The MXene has a layered structure and proved to be an excellent immobilization matrix providing good compatibility with the enzyme beta-hydroxybutyrate dehydrogenase. The MXene-based biosensor, best operated at a potential of - 0.35 V (vs. Ag/AgCl), displays a wide linear range (0.36 to 17.9 mM), a sensitivity of 0.480 muA mM(-1) cm(-2), and a low detection limit (45 muM). The biosensor was successfully applied to the determination of beta-hydroxybutyrate in (spiked) real serum samples. Graphical abstract Schematic representation of the synthesis and decoration of Mxene 2D sheets with beta-hydroxybutyrate dehydrogenase for the amperometric determination of beta-hydroxybutyric acid.