GIT2 Gene: Androgenic Regulation of White Adipose Tissue-Prostate Cancer Interactions

Abstract : Conditioned medium (CM) collected from adipocyte stromal cells (ASC) of WAT of both Glipr1+/+ and Glipr1 / adult males after surgical castration (or sham) were analyzed for presence of secreted growth promoting factors. Effects of CM from ASCs of each 4 subtypes of Glipr gene status were evaluated using normal human umbilical vein endothelial cells (HUVEC) and mouse prostate cancer cells (RM-9). CM from castrated Glipr1 / ASCs promoted the highest cell proliferation and tubule formation of HUVEC cells, and cell proliferation and invasion of mouse (RM-9) prostate cancer cells compared with ASCs-CM from both shamed Glipr1+/+ and Glipr1 / mice and castrated Glipr1+/+ male mice. Microarray assay results showed higher level of proliferin secreted in the CM-ASCs from castrated Glipr1+/+ and Glipr1 / mice compared to shamed Glipr1+/+ and Glipr1 / male mice and higher level in Glipr1 / compared to Glipr1+/+ CM-ASC. These stimulating effects of cell proliferation, tubule formation, and invasion were reduced when CM-ASC from castrated Glipr1 / ASCs was pretreated with prolierin antibody. Thus, we surmise secreted proliferin from WAT promotes cell proliferation and tubule formation of HUVEC and mouse prostate cancer cell invasion and castration induces proliferin secretion.