Quality differences between NILs of wheat variety Longmai 20 possessing HMW-GS 7OE + 8* and 17 + 18

In order to determine the genetic differences between high-molecular weight glutenin subunits (HMW-GS) 7OE + 8* and 17 + 18, HMW-GS 7OE + 8* from a Canadian extra-strong wheat cultivar Glenlea was introduced to Longmai 20 (1, 17 + 18, 5 + 10) by 6 consecutive backcrosses with biochemical marker-assisted selection. The near-isogenic lines (NILs) of Longmai 20 possessing HMW-GS 7OE + 8* and 17 + 18 were grown in the experimental field of the Crop Breeding Institute of Heilongjiang Academy of Agricultural Sciences from 2006 to 2009. The field experiments were conducted in a two-column contrast arrangement with four replicates. Compared with the NILs possessing subunits 17 + 18, the quality parameters of NILs with subunits 7OE + 8*, which include protein content, dry gluten content, ratio of wet gluten/dry gluten, ratio of Zeleny sedimentation/dry gluten, stability, maximum resistance and area of extensogram, increased by 1.5% (P = 0.06), − 0.7% (P = 0.47), − 1.9% (P < 0.01), 6.6% (P = 0.02), 11.2% (P = 0.6), 20.3% (P < 0.01) and 19.9% (P < 0.01), respectively. HMW-GS 7OE + 8* have a greater positive effect on the rheological properties of flour, such as the maximum resistance and area of extensogram, which are less influenced by environmental conditions compared to stability of Farinogram.