The establishment and verification of a model mice express enhanced green fluorescent protein for human glioma research

Objective To establish a new congenic inbred nude mice and analyzed the expression of enhanced green fluorescent protein (EGFP) in various parts of the brain, it is designed for the study of brain tumor orthotopic transplantation. Methods Female C57BL/6-Tg (CAG-EGFP) mice were backcrossed with male BALB/c nude mice for ten generations. The genotype and phenotype were identified by biochemical locus detection, skin transplantation and flow cytometry. The U87-red fluorescent protein (RFP) cells were inoculated in mice's brain to establish a human glioma model. Results In the 10th generation, T lymphocytes accounted for 0.3% of peripheral blood lymphocytes, retained the parental BALB/c nude mice of T lymphocytes lack of features; 14 biochemical loci are homozygous, H-2 phenotype haplotype (d type), success rate of skin transplantation is 100%, reached the standard of congenic inbred strain. There are differences in expression of brain tissue, cerebellar cortex, hippocampus, olfactory bulb, striatum, and neural stem cells showed moderate level of EGFP expression. The orthotopic glioma model show red brain tumor with green background. Conclusion The establishment of new strain into BALB/c inbred line stably expressing EGFP fluorescence, and lay a solid foundation for the study of human glioma orthotopic transplantation. Key words: Enhanced green fluorescent protein; Human glioma model; Congenic inbred strain; Nude mice