Response of pyrene and 1-pyrenol concentrations in weever's bile to pyrene exposure in seawater

GC/MS and fluorescence methods were used to study the responseof weever's bile to pyrene exposure in a short period of time (7 days) at concentrations of 0.1～10.0μg/dm~3 in experimental condition. The results showed that: (1)weever could strongly remove pyrene in seawater, (2) with the increase of pyreneconcentrations in seawater, weever could be induced to metabolize and eliminatepyrene in vivo, (3) the higher pyrene concentration in seawater, the higher 1-pyrenol concentration in weever's bile, (4) pyrene concentration in weever's bile were much higher than that in seawater, weever's bile could strongly concentrate pyrene from seawater, (5) the concentration of 1-pyrenol and pyrene in weever'sbile had good correlation with pyrene concentration in seawater, which could beused as biomarkers to indicate pyrene concentration in seawater.