A simple method for ordering of fragments in construction of pair restriction maps

A simple method for the ordering of fragments and the determination of relative positions of restriction sites when constructing pair restriction maps of linear and circular DNAs is described. The major advantages of the suggested approach as compared to the routine strategy of exhaustive search are as follows: (1) One does not need to begin the construction of the map by searching for the restrictases that produce the least number of fragments; (2) the method allows one to construct quite complicated maps, the main limiting parameter being the number of fragments during double digestion (no more than 10-12); and (3) construction of the map by this approach is less labor- and time-consuming than the routine strategy. 8 refs.