Application of Microsatellite Marker in Genetic Quality Detection of Inbred and Closed Colony

To analyze the genetic quality of BALB/c and ICR mice from two units.The result showed that the BALB/c mice from Unit A was genetic contaminated,some of the mice were mutated in some microsatellite loci,and BALB/c mice from Unit B was coinci- dence with the expecting result of the standard BALB/c strain.The ayerage heterozygosity and polymorphism information content of 22 microsatellite loci of ICR mice from Unit A was 0.379 and 0.308 respectively,this indicated that ICR mice from Unit A was medium genetic diversity,and the average heterozygosity and polymorphism information content of ICR mice from Unit B was 0.287 and 0.227,this showed that ICR mice from Unit B was low genetic diversity.So themicrosatellite marker can not only detect the genetic quality of inbred mice,but can also analyze the genetic polymorphism of closed colony.The microsatellite marker is a potential mark- er for monitoring the genetic quality of laboratory animals.