Silica nanoparticle-modified microcomb electrode for voltammetry detection of osteopontin with high sensitivity.

Osteosarcoma is a commonly occurring bone malignancy, and a second place for causing of cancer-death in adolescents and children. A sensitive silica nanoparticle (Si-NP) modified current-volt sensor was introduced to identify osteopontin antigen, a well-established biomarker for Osteosarcoma. Si-NP was extracted from the rice husk ash and utilized for the surface functionalization on Interdigitated microelectrodes sensing surface. Extracted Si-NP was as a spherical shape with uniform distribution, and confirmed by Field emission scanning electron microscopy (FESEM) and Field-emission transmission electron microscope (FETEM) observations. Si-NP was layered on the electrode surface through (3-Aminopropyl) triethoxysilane amine linker and antibody was immobilized on Si-NP through a glutaraldehyde linker. Osteopontin was effectively detected on this antibody attached surface and the determination limit reached to 0.6 ng/mL. Regression determination was y = 0.9366x - 1.1113; R² = 0.9331 at the linear range in between 0.3 and 5 ng/mL. In addition, control performances with non-immune antibody and albumin did not change the current-volt, showing the specific osteopontin identification. This research work brings out the easy and cost-effective method to diagnose Osteosarcoma and its causes. Sensitive silica nanoparticle modified current-volt sensor was introduced to identify osteopontin antigen, for Osteosarcoma. Silica nanoparticle was extracted from the rice husk ash and characterized, utilized for the surface functionalization on Interdigitated microelectrodes. The determination limit reached to 0.6 ng/mL at the linear range in between 0.3 and 5 ng/mL and specificity was probed by the specific osteopontin identification. This article is protected by copyright. All rights reserved.