Anti-tumor activity of Gleditsia sinensis thorns targeting angiogenesis

and emesis. In the present study, we investigated the antiobesity effects and antioxidant activity of KE-06. Methods: 3T3-L1 preadipocytes were differentiated into adipocytes with or without KE-06. After differentiation, we measured Oil Red O staining, glycerol-3-phosphate dehydrogenase (GPDH) activity and leptin production in 3T3-L1 adipocytes. In addition, we analyzed its effect on scavenging activities of 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (A BTS) and 2,2’-diphenyl-1-picrylhydrazyl (DPPH) radicals in in vitro systems. Its effect on low-density lipoprotein (LD L) oxidation was assessed by measuring production of malondialdehyde (MDA). Results: KE-06 significantly inhibited lipid accumulation and triglyceride production, and mediated GPDH, a major enzyme in the process of adipogenesis. Consistent with this, KE-06 stimulation significantly decreased the amount of leptin in 3T3-L1 adipose cells. Furthermore, KE-06 enhanced the scavenging activities on ABTS and DPPH radicals. The generation of MDA during LDL oxidation was significantly reduced by KE-06 treatment. Conclusion:Overall, our findings suggest thatKE-06has the potential for anti-adipogenic activity and antioxidant properties. Contact: Sae-Rom Yoo, k2aori@kiom.re.kr