Variation within a bread wheat cultivar for grain yield, protein content, carbon isotope discrimination and ash content

Abstract The study was undertaken to assess the variation within a bread wheat ( Triticum aestivum L.) cultivar, primarily for grain yield, and the implications for wheat breeding. During the 1998–1999 growing season, cv. Nestos was established in a non-replicated (NR-0) honeycomb experiment, in the absence of competition (11 547 plants ha −1 ). Ten high yielding (H) and 10 low yielding (L) plants were selected, the seeds of which were used to form the respective H and L lines. The 20 lines, along with their original cultivar, were evaluated in two locations either in the absence of competition (11 547 plants ha −1 ) during the 1999–2000 season or under competition (5 000 000 plants ha −1 ) during the 2000–2001 season. Results showed significant differentiation between lines for grain yield, determined both in the absence of competition at the single-plant level, i.e. yield per plant (YP), and under competition at the crop yield level, i.e. yield per plot (CY). Significant differences between lines were also found for grain protein content (PC), grain carbon isotope discrimination ( Δ ), and grain ash content (ASH), either in the absence of competition or under competition. A positive relationship was found between YP and CY ( r =0.53, P Δ or ASH as indirect selection criteria instead of grain yield was not supported by the study.