Determination of Nutritional Requirements in Rats: Variation with Time of Weight Gain Responses to Indispensable Amino Acids
1993
The Saturation Kinetics Model (SKM) can be used to describe physiological responses as functions of a limiting dietary nutrient. Physiological responses also vary with time, and, by graphing each parameter of the SKM as a function of time, the model equation can be used to produce a three-dimensional response surface, allowing the investigator to predict requirement as a function of both dietary nutrient concentration and time. To test this hypothesis, rats were fed diets containing graded levels of indispensable amino acids (0-10 g/100 g). The inhibition form of the SKM was able to predict the complete response range of weight gain, food intake, weight-specific weight gain and weight-specific food intake for each amino acid on a day-by-day basis
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