Detection of Human Serum Albumin Adsorption on Titania Surface Using Surface Plasmon Resonance Under Various pH
2011
Titania was investigated using surface plasmon resonance (SPR) in terms of protein adsorption under various pH solutions. The multilayer device consisting of a titania layer on Au was used. A flow cell was used to introduce the protein containing solutions and the rinsing solutions on the titania top layer of the multilayer device. Phosphate solutions at varying pH with and without the human serum albumin (HSA) were injected into the flow cell. The SPR angle was determined as the angle with minimum reflection intensity. The observed behaviour of the SPR angle indicated that HSA was adsorbed on the titania film surface, and the obtained SPR angles depended on pH of rinsing solution for adsorbed HSA on the titania surface.
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