Artículo original DIVERSIDAD GENÉTICA DE ESPECIES SILVESTRES DEL GÉNERONicotiana I: CARACTERIZACIÓN MEDIANTE MARCADORESBIOQUÍMICOS

The characterization of wild species of the genus Nicotiana is very important for the genetic breeding programs of Nicotiana tabacum L. because they can be a source of pest resistance genes to increase the genetic variability in the only commercial species of this genus in Cuba. The objective of this work was to characterize the genetic variability in ten species of the Nicotiana genus of the germoplasm bank in Cuba through the analysis of nine isozyme systems and total proteins. The isozymes assayed were peroxidase, polyphenol oxidase, esterase, carbonic anhidrase, superoxide dismutase, diaphorase, acid phosphatase, alkaline phosphatase and malic enzyme, as well as total proteins. Isozyme electrophoresis was performed on vertical slab