Abstract 32: LincRNA-p21 Regulates Neointima Formation and Atherosclerosis by Enhancing p53 Activity

Background: Long non-coding RNAs (lncRNAs) recently have been implicated in many biological processes and diseases. Atherosclerosis is a major risk factor for cardiovascular disease. However, the functional role of lncRNAs in atherosclerosis is largely unknown. Methods and Results: The downstream targets of p53 also been detected by un-bias mRNA array and specific qPCR. Furthermore, bioinformatics prediction, RNA-Immunoprecipitation, biotin-labeled RNA-pulldown and deletion mapping were performed to test the potential interaction between lincRNA-p21 and MDM2. Co-IP, genome-wide ChIP-seq and ChIP-qPCR were preformed to verify the possible influence on p53 transcriptional activity. We further investigated the function of lincRNA-p21 in vivo, therefore recombinant lincRNA-p21 knockdown lentivirus were injected into the injured area of carotid arteries injury mouse model. Then the expression of lincRNA-p21 in vascular samples of clinical coronary disease patients were also detected. We identified lincRNA-p21 ...