Selection of Group-Specific Phthalic Acid Esters Binding DNA Aptamers via Rationally Designed Target Immobilization and Applications for Ultrasensitive and Highly Selective Detection of Phthalic Acid Esters

Phthalic acid esters (PAEs) are ubiquitous in the environment, and some of them are recognized as endocrine disruptors that cause concerns on ecosystem functioning and public health. Due to the diversity of PAEs in the environment, there is a vital need to detect the total concentration of PAEs in a timely and low-cost way. To fulfill this requirement, it is highly desired to obtain group-specific PAE binders that are specific to the basic PAE skeleton. In this study, for the first time we have identified the group-specific PAE-binding aptamers via rationally designed target immobilization. The two target immobilization strategies were adopted to display either the phthalic ester group or the alkyl chain, respectively, at the surface of the immobilization matrix. The former enabled the rapid enrichment of aptamers after four rounds of selection. The top 100 sequences are cytosine-rich (44.7%) and differentiate from each other by only 1–4 nucleotides at limited locations. The top two aptamers all display t...