Conjugating pyrene onto PNA-based fluorescent probes for improved detection selectivity toward double-stranded siRNA.

We report on the design of new siRNA-binding fluorescent probes with the improved detection selectivity toward intact double-stranded siRNAs over single-stranded forms by the conjugation of pyrene unit into thiazole orange base surrogate-carrying peptide nucleic acid (PNA) that can simultaneously recognize the 3’-overhang and double-stranded sequences of target siRNAs.