DNA sequencing method based on acid-sensitive modified nucleotide
2015
The invention discloses a DNA sequencing method based on an acid-sensitive modified nucleotide. The method comprises steps: the acid-sensitive modified nucleotide shown in the formula (1) is employed as a reversible termination agent, and participates in a DNA chain extension reaction, and the formula (1) is shown in the specification. The acid-sensitive connection unit in the acid-sensitive modified nucleotide is fractured. The fluorescein is selected from BODIPY, rhodamine, coumarin, xanthenes, cyanin, pyrene, phthalocyanine, Alexa, a squaring dye, energy transduction generation dye or combination thereof. The base is selected from the following bases: U, T, C, A and G. Compared with the prior art, the provided extension method has a high accuracy, and has a high extension efficiency and a shearing efficiency especially when a template is a plurality of continuous same bases.
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