Effectiveness of sodium sulfite as an electron acceptor for bioenhanced treatment of salt-containing water produced from ASP flooding

The wastewater produced from alkaline-surfactant-polymer (ASP) flooding is a complex multiphase mixture that contains oil, polymers, surfactants and other pollutants and is thus a salt-containing industrial waste recalcitrant to treatments. Through laboratory tests, this study assessed the effectiveness of using sodium sulfite as an electron acceptor for a modified anaerobic baffled reactor (ABR) for removing oil, suspended solids, polymers and surfactants from salt-containing wastewater produced from ASP flooding. During the 90-day operation, the method established in this study successively removed 52.8%, 98.6%, 77.0%, 21.2% and 21.5% of the chemical oxygen demand (COD), oil, suspended solids, polymers and surfactants, respectively, from the wastewater. The changes in organic compounds in the reactor during the treatment were monitored through gas chromatography-mass spectrometry (GC-MS), and the results showed that the established method was very effective in removing alkanes, alkenes, cycloalkanes, aromatic hydrocarbons and esters, and the organic macromolecules in the wastewater were degraded to small molecules. The main bacterial species and microbial communities in the reactor were characterized using molecular biological techniques, and the results indicated that under the stress of high pH and salts, Halomonas sp. gradually dominated and played a major role in degrading hydrocarbons. The findings of this study can aid the development of a cost-effective biological system to treat the water produced from ASP flooding.