STUDY ON THE MECHANISM OF SALIDROSIDE INHIBITING CHOROIDAL NEOVASCULARIZATION

The paper observed the effects of Salidroside on the expression of various factor proteins in the hypoxia model of ARPE-19 cells induced by cobalt chloride, and the effects on the proliferation, migration, invasion and lumen formation of HUVEC cells. CoCl2 was used to induce ARPE-19 cells to establish a chemical hypoxia model. Western blot method was used to detect the effects of Salidroside on the expression of AKT, p-AKT, HIF-la and VEGF proteins in the hypoxia model. The ARPE-19 cell conditioned medium was divided into the normal group, the model group, the ranibizumab group, and different concentrations of Salidroside administration group to act on HUVEC cells, and the effects of the conditioned culture medium of each group of ARPE-19 cells on HUVEC cell viability were observed by CCK-8 method. In the cell scratch experiment, the Transwell chamber was used to observe the effects of ARPE-19 cell conditioned medium on the migration and invasion of HUVEC cells. The Matrigel lumen formation experiment was conducted to measure the effect on HUVEC cell lumen formation.  Salidroside can reduce the expressions of AKT, HIF-la and VEGF proteins in ARPE-19 cells induced by CoCl2, significantly inhibit HUVEC cell migration and invasion, and inhibit HUVEC cell lumen formation. Salidroside has a protective effect on ARPE-19 cells in CoCl2-induced hypoxia and inhibits angiogenesis at the cellular level. Salidroside Inhibiting Choroidal Neovascularization. The paper observed the effects of Salidroside on the expression of various factor proteins in the hypoxia model of ARPE-19 cells induced by cobalt chloride, and the effects on the proliferation, migration, invasion and lumen formation of HUVEC cells.