Detection of YMDD mutant HBV by mismatched-PCR and analysis of restriction fragment length polymorphism

Sera of 108 patients with chronic hepatitis B treated with lamivudine and showed positive HBV DNA(PCR)before treatment were detectedfor YMDD mutations in HBV by method of combining nested/ mismatched PCR with analysis of restriction frament lengt polyomorphism (RFLP). The above method can be applied to distinguish well YMDD wild and mutant HBV; In patients treated with lamivudine, HBV DNA furmed negative in 63 cases (58.3%). 23 (21.3%) and 22(20.4%) showed YMDD wild and nutant HBV, respectivley. Nested/mismatched PCR-RFLP can be applied to indentifying YMDD mutant HBV chlinically; lamivudine may lead of YMDD mutations in P gene of HBV in some chronic hepatitis B patients treated with lamivudine.