Development of receptor of the immunosensor for detecting SM_2 residues

In this experiment,the method of preparation and the best detecting condition of the receptor,which is the key part of immunosensor for detecting the residue of Sulfamethazine(SM2) in agricultural products were studied,meanwhile the performance of the receptor was evaluated.Aceticacid cellulose was used as carrier structure,glutaraldehyde as cross-linking agent,hexamethylen diamine as cavity preparation reagent,the perfect and standard carrier membrane was prepared,and its performance was determined by double-blind evaluation method.The selected perfect carrier membrane was applied to coat SM2 antibody.The antigen marked with HRP was coupled on the SM2 antibody membrane,then the reduced quantity of H2O2 was detected by the dissolved oxygen meter.Simultaneously,the control membrane was conducted.The results showed that there was a significant difference between the control membrane and the determining one in change of the induce electric current(P0.05),and the detection limit was 5 μg·L-1.It could be concluded that the receptor was sensitive to change of the current and could be used as the key part of the immunosensor.