Biochemical studies on the fate of aflatoxins contaminated cottonseed after treatment with ammonium hydroxide.

Treatment of contaminated cottonseed cake with NH 4 OH caused detoxification of aflatoxins found in all contamined samples. Detoxification rate for aflatoxin B 1 was higher than that of B 2 and G 2 at very low concentration (0,1% ammonium hydroxide). Aflatoxins mutagenicity test (Ames test, sensitivity test and brine shrimp test) confirmed the chemical determination obtained by fluorescence methods