Detection of organic acids in fermentation broth of Enterococci FQ15 by RP-HPLC.

Objective To research the changing trends of organic acids in fermentation broth of enterococco FQ15 at different fermention time.Method A detecting method of organic acids in fermentation broth of Enterococci FQ15 by RP-HPLC was established.The changing trends of organic acids at different fermentation time were obtained.Result The concentrations of propionic acid,pyruvic acid,lactic acid and acetic acid reached to their peak values during the decline phase of bacterial growth.The concentration of lactic acid declined obviously along with fermentation time,while the the content of acetic acid showed an escalating trend in the later phase of fermentation.Conclusion This method has a good repeatability with high accuracy.It provides a rapid analysis for studies on the variety and changing tendency of organic acids in microbial synthesis.