High-throughput drug profiling with voltage and calcium-sensitive fluorescent probes in human iPSC-derived cardiomyocytes.

We describe 96-well recording of synchronous electrical activities from spontaneously beating human induced pluripotent stem cell-derived cardiomyocyte monolayers using FluoVolt as a substitute for patch-clamp measurements, validated with reference pharmacological compounds to demonstrate the usefulness of the approach.