HPLC fingerprint chromatograms of Danshen Injectable Powder,intermediate and crude Danshen(Radix Salviae Miltiorrhizae) and correlation among them

Objective To establish the fingerprint chromatograms of crude Danshen(Radix Salviae Miltiorrhizae),intermediate and Danshen Injectable Powder.Methods The HPLC procedure was performed on the chromatographic column of Hypersil BDS C18(250 mm×4.6 mm,5 μm),and the mobile phase was acetonitrile and 1% phosphoric acid [7∶ 93(0-5 min),12∶ 88(5-7 min),12∶ 88(7-20 min),17∶ 83(20-22 min),17∶ 83(22-42 min),20∶ 80(42-60 min) and 22∶ 78(60-90 min)] in gradient elution.The detection wavelength was 281 nm,flow velocity,1.0 mL/min and temperature,30 ℃.Results The fingerprint chromatograms of crude Danshen,intermediate and Danshen Injectable Powder were established,and similarities of different batches of crude Danshen,intermediate and Danshen Injectable Powder were calculated,which were all above 0.95.The correlation among crude Danshen,intermediate and Danshen Injectable Powder was reviewed by applying the software of similarity evaluation,and the result showed that the correlation was good.Conclusion The fingerprint chromatograms had complete information under the defined HPLC condition with a good separating degree.Danshen Injectable Powder had good similarity,which indicated that quality of the products prepared under this condition was excellent.