In vivo monitoring the dynamic process of acute retinal hemorrhage and repair in zebrafish with spectral‐domain optical coherence tomography

Retina, the only light sensor in the human eye, is hidden and extremely fragile. Optimized animal models and efficient imaging techniques are very important for the study of retinopathy. In this work, the rapid retinal injury process and the long-term retinal repair process were in vivo continuously evaluated with a novel imaging technology spectral-domain optical coherence tomography (SD-OCT) in a unique animal model zebrafish. Acute retinal injury was constructed on adult zebrafish by needle injection surgery. SD-OCT imaging was carried out immediately after the mechanical injury. The retinal hemorrhage, which lasted only 5 seconds, could be visualized dynamically by SD-OCT. The process of blood clearance and retinal repair was also evaluated because SD-OCT imaging is nondestructive. Both SD-OCT imaging results and behavioral analyzing results demonstrated that zebrafish retina could be repaired by itself within 15 days, which was confirmed by the results of pathological experiment.