Lipid-based diets improve muscarinic neurotransmission in the hippocampus of transgenic APPSwe/PS1dE9 mice
2015
Transgenic APPswe/PS1dE9 mice modeling Alzheimer’s disease demonstrate ongoing accumulation
of β-amyloid fragments resulting in formation of amyloid plaques that starts at the age of
4-5 months. Buildup of β-amyloid fragments is accompanied by impairment of muscarinic transmission
that becomes detectable at this age, well before the appearance of cognitive deficits that manifest
around the age of 12 months. We have recently demonstrated that long-term feeding of trangenic mice
with specific isocaloric fish oil-based diets improves specific behavioral parameters. Now we report on the influence of
short-term feeding (3 weeks) of three isocaloric diets supplemented with Fortasyn (containing fish oil and ingredients
supporting membrane renewal), the plant sterol stigmasterol together with fish oil, and stigmasterol alone on markers of
cholinergic neurotransmission in the hippocampus of 5-month-old transgenic mice and their wild-type littermates. Transgenic
mice fed normal diet demostrated increase in ChAT activity and attenuation of carbachol-stimulated GTP-γ 35 S binding
compared to wild-type mice. None of the tested diets compared to control diet influenced the activities of ChAT,
AChE, BuChE, muscarinic receptor density or carbachol-stimulated GTP-γ 35 S binding in wild-type mice. In contrast, all
experimental diets increased the potency of carbachol in stimulating GTP-γ 35 S binding in trangenic mice to the level
found in wild-type animals. Only the Fortasyn diet increased markers of cholinergic synapses in transgenic mice. Our data
demonstrate that even short-term feeding of transgenic mice with chow containing specific lipid-based dietary supplements
can influence markers of cholinergic synapses and rectify impaired muscarinic signal transduction that develops in
transgenic mice.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
0
References
14
Citations
NaN
KQI