Inhibitory effects of microRNA-100 on migration of human bladder urothelial carcinoma cells through down-regulating S100A4 expression

Objective To explore the effects of microRNA-100(miRNA, miR-100) on S100A4 and migration in human bladder urothelial carcinoma cells. Methods We established human bladder urothelial carcinomo cell transfectants stably expressing miR-100 and SiS100A4 using lentivirus infection.The expression levels of miR-100 and S100A4 were determined by real-time quantitative reverse transcriptase-polymerase chain reaction(RT-qPCR)and Western blotting.The relationship between S100A4 and migration of EJ cells was evaluated using Transwell assays. Results S100A4 m RNA and protein expression levels were significantly decreased in human bladder urothelial carcinoma cells after transfection with lenti-miR-100(P< 0. 01).Meanwhile, silence of endogenous S100A4 by lenti-SiS100A4 could reduce the migration.The results of transwell assay showed that the number of migrating cells in high miR-100 expression group was 130. 0±3. 2, significantly lower than that in control group(450. 0±1. 5, P< 0. 01). After S100A4 down-regulation, the number of migrating cells was strongly reduced(137. 0±5. 3,P< 0. 01). Conclusion Stable expression of miR-100 reduced migration abilities via down-regulating S100A4 in human bladder urothelial carcinoma cells. Key words: microRNA-100; Human bladder urothelial carcinogenesis; S100A4; Migrate